Fulvic acid promotes extracellular anti-cancer mediators from RAW264.7 cells, causing to cancer cell death in vitro
Enhanced cell death in various human cancer cells such as Hep3B, LNCaP, and HL60. Fulvic Acid most likely stimulates immune-modulating molecules such as Nitric Oxide and induces cancer cell apoptosis.
Patients with cancer causing tumors of the esophagus had 100% success rate in preventing tumor progression into the cancerous state when treated for two years with a humic extract solution.
Yuan, Shenyuan; Fulvic Acid, 4 1988; in Application of Fulvic acid and its derivatives in the fields of agriculture and medicine; First Edition: June 1993
Outpatient medical hospital studies on thyroid tumors, some cancerous, showed that injections with a special humic extract was 90% successful in stopping tumor growth and diminishing size of tumors, with 80% of patients having complete cures.
He, Shenyi, et al; Humic acid in Jiangxi Province, 1 (1982)
Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells
A high level of serum resistin has recently been found in patients with a number of cancers, including colorectal cancer (CRC). Hence, resistin may play a role in CRC development. Fulvic acid (FA), a class of humic substances, possesses pharmacological properties. However, the effect of FA on cancer pathophysiology remains unclear. The aim of this study was to investigate the effect of resistin on the endothelial adhesion of CRC and to determine whether FA elicits an antagonistic mechanism to neutralize this resistin effect. Human HCT-116 (p53-negative) and SW-48 (p53-positive) CRC cells and human umbilical vein endothelial cells (HUVECs) were used in the experiments. Treatment of both HCT-116 and SW-48 cells with resistin increases the adhesion of both cells to HUVECs. This result indicated that p53 may not regulate this resistin effect. A mechanistic study in HCT-116 cells further showed that this resistin effect occurs via the activation of NF-κB and the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Co-treating cells with both FA and resistin revealed that FA significantly attenuated the resistin-increased NF-κB activation and ICAM-1/VCAM-1 expression and the consequent adhesion of HCT-116 cells to HUVECs. These results demonstrate the role of resistin in promoting HCT-116 cell adhesion to HUVECs and indicate that FA might be a potential candidate for the inhibition of the endothelial adhesion of CRC in response to resistin.
Shilajit induces apoptosis and inhibits proliferation via modulating reactive oxygen species in hepatic cancer cells
Bowel / Rectal Cancer – Colorectal cancers
JC virus DNA is present in the mucosa of the human colon and in colorectal cancers
These results indicate that JCV DNA can be found in colonic tissues, which raises the possibility that this virus may play a role in the chromosomal instability observed in colorectal carcinogenesis.
Shilajitin Cancer Treatment: Probable Mode of Action
Humic and fulvic acid have been reported to possess cancer preventive properties. It has been shown that these compounds can inhibit mutagenesis and have free radicals scavenging, photo-protecting, anti-inflammatory and toxic compound removing properties that can inhibit the cancer development. Shilajit is non-expensive, non-toxic compound which can be taken orally. Therefore, it is rational that future clinical studies should focus on examining the efficacy of Shilajit and its active constituents in cancer prevention as an alternative pharmacological agents. In this review, we address the use of Shilajit and its constituents for the prevention and management of cancer. Shilajit is one such compound, which has been used in Ayurveda for centuries. The humic compounds of Shilajit can be a potential cancer preventive agent. Antiinflammatory, anti- oxidative, anti-mutagenic, immuno-modulatory and several other effects of Shilajit have been already reported.
Mineral pitch (MP) induces apoptosis and inhibits proliferation via modulating reactive oxygen species in hepatic cancer cells
MP is a humic matter, shown to contain fulvic acid and humic acid, which are responsible for its biochemical activities. In some previous studies humic matter has been reported to be anticancer agent as it inhibited the cancer cell growth and induced the apoptosis . Moreover the cytotoxic properties of humic acid was accompanied the ROS production  and NO synthesis . MP has been reported to be useful in reducing inflammation, arthritis, rheumatism, pain, ulcer, anxiety, stress, and diabetes . Recently, MP also has been reported as an antiviral agent against Herpes Simplex Virus (HSV)  however, many of its other potential functions including its effects on cancer, has not been studied so far.
In this study, the anticancer property of MP was determined in hepatic cancer cells. It is an essential ethno-medicinal food, has large demand due to many therapeutic benefits in remedial recipes. Since a large population of Nepal and India customarily consume MP, the present investigation might have some implication in understanding its therapeutic significance in HCC management.
Shilajit: A Humic cure for Cancer
Shilajit and HA possess anti-inflammatory, antioxidant, antimutagenic, antitoxic, antiviral, heavy metal chelating, antitumor, apoptotic and photo-protective properties. These properties make Shilajit useful agents for cancer therapy and prevention. In addition, Shilajit has no reported side effects and can be administered as a nutritive and rejuvenating tonic and combats age related problems.
Shilajit is an ancient medicine and wonder drug of the Ayurveda used by the Hindu physicians for the treatment of several body disorders. The humic matter component of Shilajit has numerous beneficial effects on the human body. Charaka Samhita recommends Shilajit as a cure for numerous human ailments and is highly recommended by the Ayurvedic practitioners. Shilajit and its humic components have anti oxidant, antiviral, antiinflammatory, anti ulcerative, antimutagenic and heavy metal chelating properties. Therefore, Shilajit and humic compounds with these properties would possibly be a useful cancer chemopreventive agent and may be beneficial in cancer therapy. In addition, Shilajit is useful in arthritis, can function as anti fungal agent as well as nutritive tonic that help in the nutrient transfer to the deeper tissues of our body. Shilajit has immense therapeutic properties and certainly can be proved to be a boon for mankind possibly in cancer prevention and therapy.
Effects of Fulvic Acid on Different Cancer Cell Lines
Fulvic acid (FA) is known to promote electrochemical balance as a donor or a receptor possessing many biomedical functions. Nevertheless, the effect of FA on the anti-cancer activity has not been elucidated. In the current study, we first isolated FA from humus and investigated whether FA regulates immune-stimulating functions, such as production of nitric oxide (NO), in RAW 264.7 cells. Our data showed that FA slightly enhances cell viability in a dose-dependent manner and secretion of NO from RAW 264.7 cells. It upregulated the protein and mRNA expression of inducible NO synthesis (iNOS). In addition, FA enhanced the DNA-binding activity of nuclear factor-κB (NF-κB) in RAW 264.7 cells; the NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC) effectively attenuated the expression of FA-stimulated iNOS, suggesting that FA stimulates NF-κB to promote iNOS and NO production. Finally, FA-stimulated culture media (FA-CM) from RAW 264.7 cells were collected and MCA-102 fibrosarcoma cells were cultured in this media. The FA-CM augmented MCA-102 fibrosarcoma cell apoptosis; however, an NO inhibitor N(G)-monomethyl-l-arginine (NMMA) slightly inhibited the FA-CM-mediated MCA-102 fibrosarcoma cell apoptosis, which was accompanied by low levels of NO. Inthe present study, we found that FA induces the generation of NO and iNOS in RAW 264.7 cells by inducing NF-κB activation; however, NO did not significantly stimulate MCA-102 fibrosarcoma cell apoptosis in the current study. In addition, FA-CM enhanced cell death in various human cancer cells suchas Hep3B, LNCaP, and HL60. Taken together, FA most likely stimulates immune-modulating molecules such as NO and induces cancer cell apoptosis.
The Cytotoxic Effects of Humic Acid on Human Breast Cancer Cells
Humic substances are commonly found in decaying organic matter including plants, animal residues, sewage and soil. Although fulvic-acid accounts for ∼90% of all substances and its biological significance recognized for many years, there is still minimal scientific understanding the claims of its biological properties. However, these formulations contain numerous toxic elements that make their use clinically impossible. Recently, there has been development of a pure form of fulvic-acid, carbohydrate derived fulvic-acid (CHD-FA) that has been shown to be safe to use clinically and absent from environmental contaminants known to be harmful to the host. The aim of this study is to evaluate the effects of fulvic-acid on different cancer cells. Hep3B, HT29 and PC3 cells were treated different concentrations fulvic-acid for 48 and 72 h and cell proliferation was performed MTT test. Changes in the mRNA levels of apoptotic genes were also analyzed in PC3 cells. Fulvic-acid inhibited the proliferation of all the cell lines used in this study determined by MTT analysis. Specifically, Hep3B cells were found to be most sensitive for 48-hour application with 1.58–2.43 μg/μL IC50 value. Additionally, fulvic-acid significantly upregulated the apoptotic genes at mRNA levels compared to non-treated control group.
Humic acid enhances the cytotoxic effects of arsenic trioxide on human cervical cancer cells
Our results shown both As2O3 and HA-induced inhibition of cell growth, most likely by ROS-mediated cell damage and activation of the apoptosis pathway, and HA enhanced the anti-proliferative action of As2O3 in HeLa and SiHa cells, which reduced the LC50 about 57.62 or 73.52% (300 μg HA/mL) to 83.67 or 79.03% (500 μg HA/mL), respectively. This study is relevant to the development of chemotherapeutic approaches using As2O3 in treating human cervical cancer.
Antitumor effect of humus extract on murine transplantable L1210 leukemia.
Humic substances are formed during the decomposition of organic matter in humus that found in many natural environments in which organic materials and microorganisms have been present. In the present study, humus extract exhibited antitumor effect on L1210 tumor development in isogeneic DBA/2 mice with the delay of tumor formation and a significant smaller tumor mass that infer a significant increase of life span of mice. The antitumor effect was not due to direct killing of L1210 or induction of apoptosis in tumor cells by humus extract.
Cancer / Thyroid Tumors
Treating thyroid tumor with Fulvic acid
Report on ten case studies
Thyroid tumor, a commonly seen ailment, has been treated by thyroidectomy surgical procedure (thyroid gland removal).
From March 1977 to April 1980, Dr. Shenyi He and his coworkers treated 10 patients with fulvic acid. The results were successful. Follow up visits with the patients showed that in eight cases the tumor disappeared without relapse. Details are listed below:
Number of patients: 3 cases male and 7 cases female patients
Age: 17 to 36
Size and location of the growth: 3-4 cm x 4-5 cm, next to the narrow band of the thyroid gland; 7 cases leaning left, three leaning right.
Time lapse between first detection of the tumor and when treatment began: 1 week to 10 years.
Treatment: 1 case study: fulvic acid syrup; 6 case studies: fulvic acid tablets and fulvic acid injections; 3 cases: fulvic acid tablets, fulvic acid injections, and fulvic acid injection applied into tumor growth.
The female patients with thyroid tumor were under 40 years of age. Tumors of all sizes and shaped round or oval were observed and had a firmer texture than the normal gland. Its shape was better defined and slow growing. There was no pain when pressure was applied. It went up and down when food was being swallowed. Patients rarely experience any discomfort and thyroid functions were all tested normal.
Standards for evaluation of the therapeutic effects:
Cured: After three courses of treatment, the tumor disappears; Effective: After three courses of treatment, most of or part of the tumor shrinks; Not cured: After three courses of treatment, the tumor remains the same size.
Methods of treatment:
1. The regimen consisted of 20 days of three times a day intake of 10 ml syrup containing 50% fulvic acid. The same regimen is repeated another two times.
2. The regimen consists of 20 days of intramuscular injection with a 2 ml dosage containing 0.20% fulvic acid twice daily.
3. One injection in the tumor region, 4 ml of 4% fulvic acid.
4. The regimen consists of 20 days of taking fulvic acid tablets (0.3 x 4#), three times a day.
Source of material:
The preparation room of the Humic acid purification factory at Ruichang School prepares the fulvic acid syrup. People’s Hospital in Ruichang County manufactures fulvic acid tablets and the solutions for fulvic acid injections.
Analysis of Therapeutic effect:
Within this case study group: Two patients after one course of treatment found their tumor to have disappeared; four patients found their tumor to have disappeared after two courses of treatment; two found their tumor to have disappeared after three courses of treatment; one patient did not complete the treatment; one patient after one course of treatment found the size of the tumor reduced, it was followed by surgically removing the growth.
The thyroid tumor is a follicular adenomas. It is shaped like a pocket with the inner wall as shrunken follicles, some appear solid, arranged randomly, partially fiber-like, possibly due to the action of fulvic acid. Complete cure: 80%; effective: 10%; no effect: 10% (The patient treated with surgical procedures was not included in these statistics.)
Case 1: Ms. Wang, 34 years old, is a married factory worker. In March 1977 she experienced soreness in her elbows and discomfort in her neck . A growth the size of a ping-pang ball was found in her neck. She was examined as a outpatient and diagnosed as having a thyroid tumor. After being treated with fulvic acid syrup for two months, the tumor shrank and eventually disappeared. Follow-up visits showed there was no recurrence.
Case 2: Ms. Zhou is a 36 year old peasant, married. In October 1978 she discovered a growth in the front of her neck. Examination showed that the tumor had a size of 4 x 5 cm, detectable near the left side of the narrow band. It was soft to the touch. It had a defined shape and did not hurt when pressure was applied. It went up and down when food was being swallowed. She was diagnosed as having thyroid tumor. After a month’s treatment using fulvic acid injection together with fulvic acid tablets, the lump disappeared.
Case 3: Ms. Tan, 22 years old, is a teacher in a private school. In April 1977, a lump as big as an egg was found in her neck area. She felt inhibited when breathing. After three regimens with fulvic acid intramuscular injection, the lump was reduced in size. There has not since been a relapse.
Case 4: Mr. Liang, 17 years old, is a student. The growth in his neck was discovered 6 years ago. It was located in the center leaning towards to the right, its size 3 x 3 cm. Treatment started on April 2, 1979, consisting of fulvic acid injection together with fulvic acid tablets. One month later the lump began to shrink and two months later it disappeared. Follow-up visits showed there was no recurrence.
Case 5: Ms. Luo is a 30 year old married woman. For over a month she felt there was a lump in her neck and her throat felt itchy. A tumor with a size of 3 x 3 cm was detected upon examination, detectable near the left side of the narrow band. It had a medium firmness to the touch and when pressure was applied, it did not hurt. It moved up and down when food was being swallowed. After two regimens of fulvic acid treatment beginning on December 20, 1979, the lump disappeared.
Points of understanding:
1. All 10 cases were out-patient treatments. Before and after treatment, no other medicine was used. The disappearance of tumor is without doubt the results of using fulvic acid.
2. According to related reports, fulvic acid has a anti-viral effect. It heals cancer and allows the thyroid gland to be normal again.
3. The disappearance of the thyroid tumor may be related to the inhibition towards thyroid tumor cells, relating to the inhibition of its uncontrollable growth.
4. Patients feel fine during treatment, free of any discomfort.
5. The treatment is simple and the cost of medicine is low. The patients are freed of suffering from surgery and its related complications. Patients do not need to lose work time.
6. Based on limited case study, injections with syrup together with tablets give fast results.
7. To ensure best result, the patient is required to take the whole course of treatment without interruption.
Shenyi He, et al; Humic acid in Jiangxi Province, 1 (1982). In: Application of Fulvic acid and its derivatives in the fields of agriculture and medicine ; Chapter 34; First Edition: June 1993.
Cancers are Viral
Common Virus Shows Link To Brain Cancer In Children Colon, penile, uterine, cervical, liver, and numerous other cancers are also caused by viruses February 21, 2002
A study appearing today in the Journal of the National Cancer Institute, shows that the JC Virus (JCV) which infects about 65% of children by age 14, likely plays a role in the development of the most common type of malignant brain tumor found in children. This finding is consistent with many other recent reports linking viruses to cancer. The JC Virus is named after a patient, John Cunningham, from whom the virus was first isolated in the 1970’s. According to the senior author of the study, Dr. Kamel Khalili, the JC Virus “is very common,” and he went on to say that “sixty-five to 70 percent of human populations worldwide get infected with this virus by age 14, so we all basically have this virus in our bodies.” Twenty specimens of brain tumors taken from children showed evidence of the JC Virus. Dr. Khalili says that “the presence of the virus… is suggestive of a biological role for this virus in the development of these tumors.” Khalili, who is a cancer researcher at Philadelphiaís Temple University, said that earlier lab studies show that the JC Virus causes cancer in rats, mice, and brain tumors in certain monkeys. The JC virus comes from a viral group called Neutropic Polyomaviruses. It usually infects the upper respiratory system by inhalation of airborne particles just like the common cold. Polyomavirus caused respiratory infections are very common in children. The virus causes no serious disease unless a person’s immune system is weak. In patients with very weak or destroyed immune systems (such as AIDS patients, or those who are taking organ anti-rejection drugs), according to Dr. Khalili, the JC Virus can cause progressive Multifocal Leukoenchephalopathy (PML), a fatal brain disease. Dr. Khalili says that evidence suggests that JC Virus also plays a role in the development of the most common brain cancer in children, medulloblastoma. This type of cancer is diagnosed annually in about one out of every 200,000 children under the age of 15. It is a very aggressive cancer that is difficult to treat and often is fatal. Dr. Khalili says that if it can be conclusively proven that JC virus does play role in the brain cancer, then it may be possible to develop a vaccine that could help in treating the tumor or preventing its spread. Studies by Dr. L. Laghi and associates have shown that JC Polyoma Virus also causes colon cancer. In other extensive research Dr. Richard C. Hunt, at the University of South Carolina, has documented many other viruses as the cause for colon, penile, uterine, cervical, liver, and numerous other cancers. This information provides support for the idea that humic substances (fulvic acids) are a little-known and important missing link throughout the food chain. Modern agricultural practices have eliminated the humic substances from farm soil, which parallels increases in viral plant, animal, and human viral infections. Common to rich organic soil humus, fulvic acids have been shown to have unequalled value in defending plants, animals, and man against viruses of all kinds, and also in significantly increasing and balancing immunity. Of interest is also the fact that hospital studies in China showed that children with serious respiratory infections responded exceptionally well to humic – fulvic extracts when nothing else seemed to work.
Humic substances are nature’s most powerful antiviral. New studies continue to show virus – cancer connection The relationship of viruses to cancer is not too surprising, considering the mounting evidence that shows that there is a missing link in our food chain that is allowing viruses to run rampant in their attack on humans, animals, and even our food crops. What may surprise you most is that drug companies have sponsored extensive secret studies for the purpose of profiting from this dire situation, when in fact inexpensive and effective natural solutions exist. This entire website documents the relationships. Of immense interest is the fact that medical hospital studies show that difficult viral respiratory illnesses common in children are readily resolved with fulvic acid dietary supplementation. Fulvic acid is a humic extract common to rich organic humus soil and also certain ancient plant deposits. Many medical studies show that humic substances, especially fulvic acids, have the power to protect against cancer AND the related cancer causing viruses. Studies often show reversal of deadly cancers and tumors using special humic substance therapies. Many studies and extensive references exist, a few of which are referenced below. Search keywords: respiratory, tumor, virus, cancer Also see the following articles for more information: Common Virus Shows Link To Brain Cancer In Children Incurable HIV or Aids virus is destroyed by humic extracts, epidemic now has over 33 million people infected Fulvic acid’s diverse spectrum of immune responses Cancer is second leading cause of death in the US, humic extracts arrest cancer growth according to medical studies Research on the development of the medicinal applications of Fulvic acid in China